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Cas9-assisted recombineering in C. elegans: genome editing using in vivo assembly of linear DNAs

Recombineering, the use of endogenous homologous recombination systems to recombine DNA in vivo, is a commonly used technique for genome editing in microbes. Recombineering has not yet been developed for animals, where non-homology-based mechanisms have been thought to dominate DNA repair. Here, we...

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Vydáno v:Nucleic Acids Res
Hlavní autoři: Paix, Alexandre, Schmidt, Helen, Seydoux, Geraldine
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2016
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5009740/
https://ncbi.nlm.nih.gov/pubmed/27257074
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkw502
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