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RAC-tagging: Recombineering And Cas9-assisted targeting for protein tagging and conditional analyses
A fluent method for gene targeting to establish protein tagged and ligand inducible conditional loss-of-function alleles is described. We couple new recombineering applications for one-step cloning of gRNA oligonucleotides and rapid generation of short-arm (~1 kb) targeting constructs with the power...
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| Опубликовано в: : | Sci Rep |
|---|---|
| Главные авторы: | , , , , , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Nature Publishing Group
2016
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4877586/ https://ncbi.nlm.nih.gov/pubmed/27216209 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep25529 |
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