Click-electron microscopy for imaging metabolically tagged non-protein biomolecules

Electron microscopy (EM) has long been the main technique to image cell structures with nanometer resolution, but has lagged behind light microscopy in the crucial ability to make specific molecules stand out. Here we introduce “Click-EM,” a labeling technique for correlative light microscopy and EM...

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Bibliografische gegevens
Gepubliceerd in:Nat Chem Biol
Hoofdauteurs: Ngo, John T., Adams, Stephen R., Deerinck, Thomas J., Boassa, Daniela, Rodriguez-Rivera, Frances, Palida, Sakina F., Bertozzi, Carolyn R., Ellisman, Mark H., Tsien, Roger Y.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 2016
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Article
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4871776/
https://ncbi.nlm.nih.gov/pubmed/27110681
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nchembio.2076
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