Click-electron microscopy for imaging metabolically tagged non-protein biomolecules

Electron microscopy (EM) has long been the main technique to image cell structures with nanometer resolution, but has lagged behind light microscopy in the crucial ability to make specific molecules stand out. Here we introduce “Click-EM,” a labeling technique for correlative light microscopy and EM...

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Udgivet i:Nat Chem Biol
Main Authors: Ngo, John T., Adams, Stephen R., Deerinck, Thomas J., Boassa, Daniela, Rodriguez-Rivera, Frances, Palida, Sakina F., Bertozzi, Carolyn R., Ellisman, Mark H., Tsien, Roger Y.
Format: Artigo
Sprog:Inglês
Udgivet: 2016
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4871776/
https://ncbi.nlm.nih.gov/pubmed/27110681
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nchembio.2076
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