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Click-electron microscopy for imaging metabolically tagged non-protein biomolecules

Electron microscopy (EM) has long been the main technique to image cell structures with nanometer resolution, but has lagged behind light microscopy in the crucial ability to make specific molecules stand out. Here we introduce “Click-EM,” a labeling technique for correlative light microscopy and EM...

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Detalhes bibliográficos
Publicado no:Nat Chem Biol
Main Authors: Ngo, John T., Adams, Stephen R., Deerinck, Thomas J., Boassa, Daniela, Rodriguez-Rivera, Frances, Palida, Sakina F., Bertozzi, Carolyn R., Ellisman, Mark H., Tsien, Roger Y.
Formato: Artigo
Idioma:Inglês
Publicado em: 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4871776/
https://ncbi.nlm.nih.gov/pubmed/27110681
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nchembio.2076
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