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Electron microscopy using the genetically encoded APEX2 tag in cultured mammalian cells

Electron microscopy (EM) is the premiere technique for high-resolution imaging of cellular ultrastructure. Unambiguous identification of specific proteins or cellular compartments in electron micrographs, however, remains challenging because of difficulties in delivering electron-dense contrast agen...

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Detalhes bibliográficos
Publicado no:Nat Protoc
Main Authors: Martell, Jeffrey D, Deerinck, Thomas J, Lam, Stephanie S, Ellisman, Mark H, Ting, Alice Y
Formato: Artigo
Idioma:Inglês
Publicado em: 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5851282/
https://ncbi.nlm.nih.gov/pubmed/28796234
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2017.065
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