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Directed evolution of APEX2 for electron microscopy and proteomics
APEX is an engineered peroxidase that functions both as an electron microscopy tag, and as a promiscuous labeling enzyme for live-cell proteomics. Because the limited sensitivity of APEX precludes applications requiring low APEX expression, we used yeast display evolution to improve its catalytic ef...
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| Pubblicato in: | Nat Methods |
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| Autori principali: | , , , , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
2014
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4296904/ https://ncbi.nlm.nih.gov/pubmed/25419960 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.3179 |
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