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biGBac enables rapid gene assembly for the expression of large multisubunit protein complexes
Analyses of protein complexes are facilitated by methods that enable the generation of recombinant complexes via coexpression of their subunits from multigene DNA constructs. However, low experimental throughput limits the generation of such constructs in parallel. Here we describe a method that all...
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| Vydáno v: | Proc Natl Acad Sci U S A |
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| Hlavní autoři: | , , , , , , , , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
National Academy of Sciences
2016
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4868461/ https://ncbi.nlm.nih.gov/pubmed/27114506 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1604935113 |
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