biGBac enables rapid gene assembly for the expression of large multisubunit protein complexes

Analyses of protein complexes are facilitated by methods that enable the generation of recombinant complexes via coexpression of their subunits from multigene DNA constructs. However, low experimental throughput limits the generation of such constructs in parallel. Here we describe a method that all...

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Detalles Bibliográficos
Publicado en:Proc Natl Acad Sci U S A
Autores principales: Weissmann, Florian, Petzold, Georg, VanderLinden, Ryan, Huis in 't Veld, Pim J., Brown, Nicholas G., Lampert, Fabienne, Westermann, Stefan, Stark, Holger, Schulman, Brenda A., Peters, Jan-Michael
Formato: Artigo
Lenguaje:Inglês
Publicado: National Academy of Sciences 2016
Materias:
PNAS Plus
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4868461/
https://ncbi.nlm.nih.gov/pubmed/27114506
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1604935113
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