biGBac enables rapid gene assembly for the expression of large multisubunit protein complexes

Analyses of protein complexes are facilitated by methods that enable the generation of recombinant complexes via coexpression of their subunits from multigene DNA constructs. However, low experimental throughput limits the generation of such constructs in parallel. Here we describe a method that all...

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Bibliografiska uppgifter
I publikationen:Proc Natl Acad Sci U S A
Huvudupphovsmän: Weissmann, Florian, Petzold, Georg, VanderLinden, Ryan, Huis in 't Veld, Pim J., Brown, Nicholas G., Lampert, Fabienne, Westermann, Stefan, Stark, Holger, Schulman, Brenda A., Peters, Jan-Michael
Materialtyp: Artigo
Språk:Inglês
Publicerad: National Academy of Sciences 2016
Ämnen:
PNAS Plus
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC4868461/
https://ncbi.nlm.nih.gov/pubmed/27114506
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1604935113
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