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biGBac enables rapid gene assembly for the expression of large multisubunit protein complexes

Analyses of protein complexes are facilitated by methods that enable the generation of recombinant complexes via coexpression of their subunits from multigene DNA constructs. However, low experimental throughput limits the generation of such constructs in parallel. Here we describe a method that all...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Weissmann, Florian, Petzold, Georg, VanderLinden, Ryan, Huis in 't Veld, Pim J., Brown, Nicholas G., Lampert, Fabienne, Westermann, Stefan, Stark, Holger, Schulman, Brenda A., Peters, Jan-Michael
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2016
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4868461/
https://ncbi.nlm.nih.gov/pubmed/27114506
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1604935113
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