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biGBac enables rapid gene assembly for the expression of large multisubunit protein complexes

Analyses of protein complexes are facilitated by methods that enable the generation of recombinant complexes via coexpression of their subunits from multigene DNA constructs. However, low experimental throughput limits the generation of such constructs in parallel. Here we describe a method that all...

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Bibliografski detalji
Izdano u:Proc Natl Acad Sci U S A
Glavni autori: Weissmann, Florian, Petzold, Georg, VanderLinden, Ryan, Huis in 't Veld, Pim J., Brown, Nicholas G., Lampert, Fabienne, Westermann, Stefan, Stark, Holger, Schulman, Brenda A., Peters, Jan-Michael
Format: Artigo
Jezik:Inglês
Izdano: National Academy of Sciences 2016
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4868461/
https://ncbi.nlm.nih.gov/pubmed/27114506
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1604935113
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