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Mechanistic Investigation of the Radical S-Adenosyl-l-methionine (SAM) Enzyme DesII Using Fluorinated Analogs

DesII is a radical S-adenosyl-l-methionine (SAM) enzyme that can act as a deaminase or a dehydrogenase depending on the nature of its TDP-sugar substrate. Previous work has implicated a substrate-derived, C3-centered α-hydroxyalkyl radical as a key intermediate during catalysis. Although deprotonati...

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Podrobná bibliografie
Vydáno v:J Am Chem Soc
Hlavní autoři: Lin, Geng-Min, Choi, Sei-Hyun, Ruszczycky, Mark W., Liu, Hung-wen
Médium: Artigo
Jazyk:Inglês
Vydáno: 2015
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4862307/
https://ncbi.nlm.nih.gov/pubmed/25826575
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jacs.5b02545
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