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Pooling across cells to normalize single-cell RNA sequencing data with many zero counts

Normalization of single-cell RNA sequencing data is necessary to eliminate cell-specific biases prior to downstream analyses. However, this is not straightforward for noisy single-cell data where many counts are zero. We present a novel approach where expression values are summed across pools of cel...

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Veröffentlicht in:Genome Biol
Hauptverfasser: L. Lun, Aaron T., Bach, Karsten, Marioni, John C.
Format: Artigo
Sprache:Inglês
Veröffentlicht: BioMed Central 2016
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4848819/
https://ncbi.nlm.nih.gov/pubmed/27122128
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-016-0947-7
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