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Detection and removal of barcode swapping in single-cell RNA-seq data

Barcode swapping results in the mislabelling of sequencing reads between multiplexed samples on patterned flow-cell Illumina sequencing machines. This may compromise the validity of numerous genomic assays; however, the severity and consequences of barcode swapping remain poorly understood. We have...

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Detalhes bibliográficos
Publicado no:Nat Commun
Main Authors: Griffiths, Jonathan A., Richard, Arianne C., Bach, Karsten, Lun, Aaron T. L., Marioni, John C.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6039488/
https://ncbi.nlm.nih.gov/pubmed/29991676
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-018-05083-x
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