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Studying the regulation of endosomal cAMP production in GPCR signaling

We describe methods based on live cell fluorescent microscopy and mass spectrometry to characterize the mechanism of endosomal cAMP production and its regulation using the parathyroid hormone (PTH) type 1 receptor as a prime example. These methods permit to measure rapid changes of cAMP levels in re...

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Publicat a:Methods Cell Biol
Autors principals: Gidon, Alexandre, Feinstein, Timothy N., Xiao, Kunhong, Vilardaga, Jean-Pierre
Format: Artigo
Idioma:Inglês
Publicat: 2015
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4848751/
https://ncbi.nlm.nih.gov/pubmed/26928541
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mcb.2015.10.007
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