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Studying the regulation of endosomal cAMP production in GPCR signaling
We describe methods based on live cell fluorescent microscopy and mass spectrometry to characterize the mechanism of endosomal cAMP production and its regulation using the parathyroid hormone (PTH) type 1 receptor as a prime example. These methods permit to measure rapid changes of cAMP levels in re...
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| Veröffentlicht in: | Methods Cell Biol |
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| Hauptverfasser: | , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2015
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4848751/ https://ncbi.nlm.nih.gov/pubmed/26928541 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mcb.2015.10.007 |
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