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Studying the regulation of endosomal cAMP production in GPCR signaling

We describe methods based on live cell fluorescent microscopy and mass spectrometry to characterize the mechanism of endosomal cAMP production and its regulation using the parathyroid hormone (PTH) type 1 receptor as a prime example. These methods permit to measure rapid changes of cAMP levels in re...

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Pubblicato in:Methods Cell Biol
Autori principali: Gidon, Alexandre, Feinstein, Timothy N., Xiao, Kunhong, Vilardaga, Jean-Pierre
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2015
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4848751/
https://ncbi.nlm.nih.gov/pubmed/26928541
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mcb.2015.10.007
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