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Studying the regulation of endosomal cAMP production in GPCR signaling

We describe methods based on live cell fluorescent microscopy and mass spectrometry to characterize the mechanism of endosomal cAMP production and its regulation using the parathyroid hormone (PTH) type 1 receptor as a prime example. These methods permit to measure rapid changes of cAMP levels in re...

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Bibliografische gegevens
Gepubliceerd in:Methods Cell Biol
Hoofdauteurs: Gidon, Alexandre, Feinstein, Timothy N., Xiao, Kunhong, Vilardaga, Jean-Pierre
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 2015
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4848751/
https://ncbi.nlm.nih.gov/pubmed/26928541
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mcb.2015.10.007
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