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Studying the regulation of endosomal cAMP production in GPCR signaling

We describe methods based on live cell fluorescent microscopy and mass spectrometry to characterize the mechanism of endosomal cAMP production and its regulation using the parathyroid hormone (PTH) type 1 receptor as a prime example. These methods permit to measure rapid changes of cAMP levels in re...

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Detalles Bibliográficos
Publicado en:Methods Cell Biol
Main Authors: Gidon, Alexandre, Feinstein, Timothy N., Xiao, Kunhong, Vilardaga, Jean-Pierre
Formato: Artigo
Idioma:Inglês
Publicado: 2015
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4848751/
https://ncbi.nlm.nih.gov/pubmed/26928541
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mcb.2015.10.007
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