Light-induced gene expression with photocaged IPTG for induction profiling in a high-throughput screening system

BACKGROUND: Inducible expression systems are frequently used for the production of heterologous proteins. Achieving maximum product concentrations requires induction profiling, namely the optimization of induction time and inducer concentration. However, the respective experiments can be very labori...

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Publicado en:Microb Cell Fact
Autores principales: Wandrey, Georg, Bier, Claus, Binder, Dennis, Hoffmann, Kyra, Jaeger, Karl-Erich, Pietruszka, Jörg, Drepper, Thomas, Büchs, Jochen
Formato: Artigo
Lenguaje:Inglês
Publicado: BioMed Central 2016
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Research
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4842301/
https://ncbi.nlm.nih.gov/pubmed/27107964
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-016-0461-3
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