Light-induced gene expression with photocaged IPTG for induction profiling in a high-throughput screening system

BACKGROUND: Inducible expression systems are frequently used for the production of heterologous proteins. Achieving maximum product concentrations requires induction profiling, namely the optimization of induction time and inducer concentration. However, the respective experiments can be very labori...

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Veröffentlicht in:Microb Cell Fact
Hauptverfasser: Wandrey, Georg, Bier, Claus, Binder, Dennis, Hoffmann, Kyra, Jaeger, Karl-Erich, Pietruszka, Jörg, Drepper, Thomas, Büchs, Jochen
Format: Artigo
Sprache:Inglês
Veröffentlicht: BioMed Central 2016
Schlagworte:
Research
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4842301/
https://ncbi.nlm.nih.gov/pubmed/27107964
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-016-0461-3
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