Light-induced gene expression with photocaged IPTG for induction profiling in a high-throughput screening system

BACKGROUND: Inducible expression systems are frequently used for the production of heterologous proteins. Achieving maximum product concentrations requires induction profiling, namely the optimization of induction time and inducer concentration. However, the respective experiments can be very labori...

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Detaylı Bibliyografya
Yayımlandı:Microb Cell Fact
Asıl Yazarlar: Wandrey, Georg, Bier, Claus, Binder, Dennis, Hoffmann, Kyra, Jaeger, Karl-Erich, Pietruszka, Jörg, Drepper, Thomas, Büchs, Jochen
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: BioMed Central 2016
Konular:
Research
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC4842301/
https://ncbi.nlm.nih.gov/pubmed/27107964
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-016-0461-3
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