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Probing unnatural amino acid integration into enhanced green fluorescent protein by genetic code expansion with a high-throughput screening platform

BACKGROUND: Genetic code expansion has developed into an elegant tool to incorporate unnatural amino acids (uAA) at predefined sites in the protein backbone in response to an amber codon. However, recombinant production and yield of uAA comprising proteins are challenged due to the additional transl...

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Dades bibliogràfiques
Publicat a:J Biol Eng
Autors principals: Wandrey, Georg, Wurzel, Joel, Hoffmann, Kyra, Ladner, Tobias, Büchs, Jochen, Meinel, Lorenz, Lühmann, Tessa
Format: Artigo
Idioma:Inglês
Publicat: BioMed Central 2016
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5045631/
https://ncbi.nlm.nih.gov/pubmed/27733867
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13036-016-0031-6
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