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Probing unnatural amino acid integration into enhanced green fluorescent protein by genetic code expansion with a high-throughput screening platform
BACKGROUND: Genetic code expansion has developed into an elegant tool to incorporate unnatural amino acids (uAA) at predefined sites in the protein backbone in response to an amber codon. However, recombinant production and yield of uAA comprising proteins are challenged due to the additional transl...
Αποθηκεύτηκε σε:
| Τόπος έκδοσης: | J Biol Eng |
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| Κύριοι συγγραφείς: | , , , , , , |
| Μορφή: | Artigo |
| Γλώσσα: | Inglês |
| Έκδοση: |
BioMed Central
2016
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| Θέματα: | |
| Διαθέσιμο Online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5045631/ https://ncbi.nlm.nih.gov/pubmed/27733867 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13036-016-0031-6 |
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