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Light-induced gene expression with photocaged IPTG for induction profiling in a high-throughput screening system

BACKGROUND: Inducible expression systems are frequently used for the production of heterologous proteins. Achieving maximum product concentrations requires induction profiling, namely the optimization of induction time and inducer concentration. However, the respective experiments can be very labori...

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Pubblicato in:Microb Cell Fact
Autori principali: Wandrey, Georg, Bier, Claus, Binder, Dennis, Hoffmann, Kyra, Jaeger, Karl-Erich, Pietruszka, Jörg, Drepper, Thomas, Büchs, Jochen
Natura: Artigo
Lingua:Inglês
Pubblicazione: BioMed Central 2016
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4842301/
https://ncbi.nlm.nih.gov/pubmed/27107964
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-016-0461-3
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