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Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications

The detection of double-stranded (ds) DNA by SYBR Green I (SG) is important in many molecular biology methods including gel electrophoresis, dsDNA quantification in solution and real-time PCR. Biophysical studies at defined dye/base pair ratios (dbprs) were used to determine the structure–property r...

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Autores principales: Zipper, Hubert, Brunner, Herwig, Bernhagen, Jürgen, Vitzthum, Frank
Formato: Artigo
Lenguaje:Inglês
Publicado: Oxford University Press 2004
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC484200/
https://ncbi.nlm.nih.gov/pubmed/15249599
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh101
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