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Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications
The detection of double-stranded (ds) DNA by SYBR Green I (SG) is important in many molecular biology methods including gel electrophoresis, dsDNA quantification in solution and real-time PCR. Biophysical studies at defined dye/base pair ratios (dbprs) were used to determine the structure–property r...
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| Главные авторы: | , , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Oxford University Press
2004
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC484200/ https://ncbi.nlm.nih.gov/pubmed/15249599 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh101 |
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