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Amplifiable DNA from Gram-negative and Gram-positive bacteria by a low strength pulsed electric field method

An efficient electric field-based procedure for cell disruption and DNA isolation is described. Isoosmotic suspensions of Gram-negative and Gram-positive bacteria were treated with pulsed electric fields of <60 V/cm. Pulses had an exponential decay waveform with a time constant of 3.4 µs. DNA yie...

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Hlavní autoři: Vitzthum, Frank, Geiger, Georg, Bisswanger, Hans, Elkine, Bentsian, Brunner, Herwig, Bernhagen, Jürgen
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2000
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC102841/
https://ncbi.nlm.nih.gov/pubmed/10734214
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