Amplifiable DNA from Gram-negative and Gram-positive bacteria by a low strength pulsed electric field method

An efficient electric field-based procedure for cell disruption and DNA isolation is described. Isoosmotic suspensions of Gram-negative and Gram-positive bacteria were treated with pulsed electric fields of <60 V/cm. Pulses had an exponential decay waveform with a time constant of 3.4 µs. DNA yield...

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Bibliografische Detailangaben

Veröffentlicht in:	Nucleic Acids Res
Hauptverfasser:	Vitzthum, Frank, Geiger, Georg, Bisswanger, Hans, Elkine, Bentsian, Brunner, Herwig, Bernhagen, Jürgen
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Oxford University Press 2000
Schlagworte:	NAR Methods Online
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC102841/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10734214/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/28.8.e37
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