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Mechanisms underlying the impact of humic acids on DNA quantification by SYBR Green I and consequences for the analysis of soils and aquatic sediments

DNA quantification of soils and sediments is useful for the investigation of microbial communities and for the acquisition of their genomes that are exploited for the production of natural products. However, in such samples DNA quantification is impaired by humic acids (HA). Due to its lack of speci...

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Hlavní autoři: Zipper, Hubert, Buta, Christiane, Lämmle, Katrin, Brunner, Herwig, Bernhagen, Jürgen, Vitzthum, Frank
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2003
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC152824/
https://ncbi.nlm.nih.gov/pubmed/12655027
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