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Demonstration of preferential binding of SYBR Green I to specific DNA fragments in real-time multiplex PCR

SYBR Green I (SG) is widely used in real-time PCR applications as an intercalating dye and is included in many commercially available kits at undisclosed concentrations. Binding of SG to double-stranded DNA is non-specific and additional testing, such as DNA melting curve analysis, is required to co...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Giglio, Steven, Monis, Paul T., Saint, Christopher P.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Oxford University Press 2003
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC275573/
https://ncbi.nlm.nih.gov/pubmed/14602929
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gng135
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