Demonstration of preferential binding of SYBR Green I to specific DNA fragments in real-time multiplex PCR
SYBR Green I (SG) is widely used in real-time PCR applications as an intercalating dye and is included in many commercially available kits at undisclosed concentrations. Binding of SG to double-stranded DNA is non-specific and additional testing, such as DNA melting curve analysis, is required to co...
Uloženo v:
| Vydáno v: | Nucleic Acids Res |
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| Hlavní autoři: | , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Oxford University Press
2003
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC275573/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/14602929/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gng135 |
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