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Multiplexed tandem PCR: gene profiling from small amounts of RNA using SYBR Green detection
Multiplexed tandem PCR (MT-PCR) is a process for highly multiplexed gene expression profiling. In the first step, multiple primer pairs are added to the RNA to be analysed together with reverse transcriptase and Taq DNA polymerase. Following reverse transcription, the multiplexed amplicons are simul...
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| Auteurs principaux: | , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Oxford University Press
2005
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1298933/ https://ncbi.nlm.nih.gov/pubmed/16314310 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gni182 |
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