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Rapid kinetic methods to dissect steroidogenic cytochrome P450 reaction mechanisms

All cytochrome P450 enzyme reactions involve a catalytic cycle with several discreet physical or chemical steps. This cycle ends with the formation of the reactive heme iron-oxygen complex, which oxygenates substrate. While the steps might be very similar for each P450 enzyme, the rates of each step...

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Detalles Bibliográficos
Publicado en:J Steroid Biochem Mol Biol
Main Authors: Yoshimoto, Francis K., Auchus, Richard J.
Formato: Artigo
Idioma:Inglês
Publicado: 2015
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4841756/
https://ncbi.nlm.nih.gov/pubmed/26472553
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jsbmb.2015.10.005
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