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Quantitative evaluation of first, second, and third generation hairpin systems reveals the limit of mammalian vector-based RNAi

Incorporating miRNA-like features into vector-based hairpin scaffolds has been shown to augment small RNA processing and RNAi efficiency. Therefore, defining an optimal, native hairpin context may obviate a need for hairpin-specific targeting design schemes, which confound the movement of functional...

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Detalhes bibliográficos
Publicado no:RNA Biol
Main Authors: Watanabe, Colin, Cuellar, Trinna L., Haley, Benjamin
Formato: Artigo
Idioma:Inglês
Publicado em: Taylor & Francis 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4829305/
https://ncbi.nlm.nih.gov/pubmed/26786363
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1080/15476286.2015.1128062
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