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High-Throughput Construction of Intron-Containing Hairpin RNA Vectors for RNAi in Plants
With the wide use of double-stranded RNA interference (RNAi) for the analysis of gene function in plants, a high-throughput system for making hairpin RNA (hpRNA) constructs is in great demand. Here, we describe a novel restriction-ligation approach that provides a simple but efficient construction o...
में बचाया:
| मुख्य लेखकों: | , , , , , |
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| स्वरूप: | Artigo |
| भाषा: | Inglês |
| प्रकाशित: |
Public Library of Science
2012
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| विषय: | |
| ऑनलाइन पहुंच: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3364983/ https://ncbi.nlm.nih.gov/pubmed/22675447 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0038186 |
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