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Intensive RNAi with lentiviral vectors in mammalian cells

RNAi is a powerful technology for analyzing gene function in human cells. However, its utility can be compromised by inadequate knockdown of the target mRNA or by interpretation of effects without rigorous controls. We review lentiviral vector-based methods that enable transient or stable knockdowns...

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Detalhes bibliográficos
Main Authors: Miest, Tanner, Saenz, Dyana, Meehan, Anne, Llano, Manuel, Poeschla, Eric M.
Formato: Artigo
Idioma:Inglês
Publicado em: 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2879882/
https://ncbi.nlm.nih.gov/pubmed/19041944
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2008.11.001
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