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Intensive RNAi with lentiviral vectors in mammalian cells

RNAi is a powerful technology for analyzing gene function in human cells. However, its utility can be compromised by inadequate knockdown of the target mRNA or by interpretation of effects without rigorous controls. We review lentiviral vector-based methods that enable transient or stable knockdowns...

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Bibliografiska uppgifter
Huvudupphovsmän: Miest, Tanner, Saenz, Dyana, Meehan, Anne, Llano, Manuel, Poeschla, Eric M.
Materialtyp: Artigo
Språk:Inglês
Publicerad: 2008
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC2879882/
https://ncbi.nlm.nih.gov/pubmed/19041944
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2008.11.001
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