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Transfer of the bacterial gene for cytosine deaminase to mammalian cells confers lethal sensitivity to 5-fluorocytosine: a negative selection system.

Expression of the bacterial gene for cytosine deaminase (CD; EC 3.5.4.1) in mammalian cells was evaluated as a negative selection system or suicide vector for potential use in gene transfer studies and therapies. Mammalian cells, unlike certain bacteria and fungi, do not contain the enzyme CD and do...

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Autores principales: Mullen, C A, Kilstrup, M, Blaese, R M
Formato: Artigo
Lenguaje:Inglês
Publicado: 1992
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC48169/
https://ncbi.nlm.nih.gov/pubmed/1729703
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