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Structure of the eukaryotic replicative CMG helicase and pumpjack motion

The CMG helicase is composed of Cdc45, Mcm2-7 and GINS. Here we report the structure of the S. cerevisiae CMG determined by cryo-EM at a resolution of 3.7–4.8 Å. The structure reveals that GINS and Cdc45 scaffold the N-tier of the helicase while enabling motion of the AAA+ C-tier. CMG exists in two...

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發表在:Nat Struct Mol Biol
Main Authors: Yuan, Zuanning, Bai, Lin, Sun, Jingchuan, Georgescu, Roxana, Liu, Jun, O’Donnell, Michael E., Li, Huilin
格式: Artigo
語言:Inglês
出版: 2016
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC4812828/
https://ncbi.nlm.nih.gov/pubmed/26854665
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nsmb.3170
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