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CRISPR library designer (CLD): software for multispecies design of single guide RNA libraries

BACKGROUND: Genetic screens using CRISPR/Cas9 are a powerful method for the functional analysis of genomes. RESULTS: Here we describe CRISPR library designer (CLD), an integrated bioinformatics application for the design of custom single guide RNA (sgRNA) libraries for all organisms with annotated g...

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Bibliografski detalji
Izdano u:Genome Biol
Glavni autori: Heigwer, Florian, Zhan, Tianzuo, Breinig, Marco, Winter, Jan, Brügemann, Dirk, Leible, Svenja, Boutros, Michael
Format: Artigo
Jezik:Inglês
Izdano: BioMed Central 2016
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4807595/
https://ncbi.nlm.nih.gov/pubmed/27013184
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-016-0915-2
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