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Crispr-mediated Gene Targeting of Human Induced Pluripotent Stem Cells

CRISPR / Cas9 nuclease systems can create double-stranded DNA breaks at specific sequences to efficiently and precisely disrupt, excise, mutate, insert, or replace genes. However, human embryonic stem or induced pluripotent stem cells (iPSCs) are more difficult to transfect and less resilient to DNA...

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書目詳細資料
發表在:Curr Protoc Stem Cell Biol
Main Authors: Byrne, Susan M., Church, George M.
格式: Artigo
語言:Inglês
出版: 2015
主題:
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC4772967/
https://ncbi.nlm.nih.gov/pubmed/26949444
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/9780470151808.sc05a08s35
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