Gene targeting of a disease-related gene in human induced pluripotent stem and embryonic stem cells

We report here homologous recombination (HR)-mediated gene targeting of two different genes in human iPS and ES cells. HR-mediated correction of a chromosomally-integrated mutant GFP reporter gene reaches efficiencies of 0.14%-0.24% in both cell types transfected by donor DNA with plasmids expressin...

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Detalhes bibliográficos
Main Authors: Zou, Jizhong, Maeder, Morgan L., Mali, Prashant, Pruett-Miller, Shondra M, Thibodeau-Beganny, Stacey, Chou, Bin-Kuan, Chen, Guibin, Ye, Zhaohui, Park, In-Hyun, Daley, George Q., Porteus, Matthew H., Joung, J. Keith, Cheng, Linzhao
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2720132/
https://ncbi.nlm.nih.gov/pubmed/19540188
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.stem.2009.05.023
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