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Crispr-mediated Gene Targeting of Human Induced Pluripotent Stem Cells
CRISPR / Cas9 nuclease systems can create double-stranded DNA breaks at specific sequences to efficiently and precisely disrupt, excise, mutate, insert, or replace genes. However, human embryonic stem or induced pluripotent stem cells (iPSCs) are more difficult to transfect and less resilient to DNA...
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| Published in: | Curr Protoc Stem Cell Biol |
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| Main Authors: | , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
2015
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4772967/ https://ncbi.nlm.nih.gov/pubmed/26949444 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/9780470151808.sc05a08s35 |
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