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Directed evolution of G protein-coupled receptors in yeast for higher functional production in eukaryotic expression hosts

Despite recent successes, many G protein-coupled receptors (GPCRs) remained refractory to detailed molecular studies due to insufficient production yields, even in the most sophisticated eukaryotic expression systems. Here we introduce a robust method employing directed evolution of GPCRs in yeast t...

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Bibliografski detalji
Izdano u:Sci Rep
Glavni autori: Schütz, Marco, Schöppe, Jendrik, Sedlák, Erik, Hillenbrand, Matthias, Nagy-Davidescu, Gabriela, Ehrenmann, Janosch, Klenk, Christoph, Egloff, Pascal, Kummer, Lutz, Plückthun, Andreas
Format: Artigo
Jezik:Inglês
Izdano: Nature Publishing Group 2016
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4766470/
https://ncbi.nlm.nih.gov/pubmed/26911446
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep21508
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