Dithiothreitol (DTT) Acts as a Specific, UV-inducible Cross-linker in Elucidation of Protein–RNA Interactions

Protein–RNA cross-linking by UV irradiation at 254 nm wavelength has been established as an unbiased method to identify proteins in direct contact with RNA, and has been successfully applied to investigate the spatial arrangement of protein and RNA in large macromolecular assemblies, e.g. ribonucleo...

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Publicado en:Mol Cell Proteomics
Autores principales: Zaman, Uzma, Richter, Florian M., Hofele, Romina, Kramer, Katharina, Sachsenberg, Timo, Kohlbacher, Oliver, Lenz, Christof, Urlaub, Henning
Formato: Artigo
Lenguaje:Inglês
Publicado: The American Society for Biochemistry and Molecular Biology 2015
Materias:
Research
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4762631/
https://ncbi.nlm.nih.gov/pubmed/26450613
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.M115.052795
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