Dithiothreitol (DTT) Acts as a Specific, UV-inducible Cross-linker in Elucidation of Protein–RNA Interactions

Protein–RNA cross-linking by UV irradiation at 254 nm wavelength has been established as an unbiased method to identify proteins in direct contact with RNA, and has been successfully applied to investigate the spatial arrangement of protein and RNA in large macromolecular assemblies, e.g. ribonucleo...

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Publicat a:Mol Cell Proteomics
Autors principals: Zaman, Uzma, Richter, Florian M., Hofele, Romina, Kramer, Katharina, Sachsenberg, Timo, Kohlbacher, Oliver, Lenz, Christof, Urlaub, Henning
Format: Artigo
Idioma:Inglês
Publicat: The American Society for Biochemistry and Molecular Biology 2015
Matèries:
Research
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4762631/
https://ncbi.nlm.nih.gov/pubmed/26450613
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.M115.052795
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