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Dithiothreitol (DTT) Acts as a Specific, UV-inducible Cross-linker in Elucidation of Protein–RNA Interactions
Protein–RNA cross-linking by UV irradiation at 254 nm wavelength has been established as an unbiased method to identify proteins in direct contact with RNA, and has been successfully applied to investigate the spatial arrangement of protein and RNA in large macromolecular assemblies, e.g. ribonucleo...
Gespeichert in:
| Veröffentlicht in: | Mol Cell Proteomics |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
The American Society for Biochemistry and Molecular
Biology
2015
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4762631/ https://ncbi.nlm.nih.gov/pubmed/26450613 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.M115.052795 |
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