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shRNA target prediction informed by comprehensive enquiry (SPICE): a supporting system for high-throughput screening of shRNA library

RNA interference (RNAi) screening is extensively used in the field of reverse genetics. RNAi libraries constructed using random oligonucleotides have made this technology affordable. However, the new methodology requires exploration of the RNAi target gene information after screening because the RNA...

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Dettagli Bibliografici
Pubblicato in:EURASIP J Bioinform Syst Biol
Autori principali: Kamatuka, Kenta, Hattori, Masahiro, Sugiyama, Tomoyasu
Natura: Artigo
Lingua:Inglês
Pubblicazione: Springer International Publishing 2016
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4761003/
https://ncbi.nlm.nih.gov/pubmed/26941783
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13637-016-0039-8
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