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A primer on using pooled shRNA libraries for functional genomic screens

The discovery of RNA interference (RNAi) has revolutionized genetic analysis in mammalian cells. Loss-of-function RNAi screens enable rapid, functional annotation of the genome. Of the various RNAi approaches, pooled shRNA libraries have received considerable attention because of their versatility....

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Hu, Guang, Luo, Ji
Format: Artigo
Sprache:Inglês
Veröffentlicht: Oxford University Press 2012
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3263926/
https://ncbi.nlm.nih.gov/pubmed/22271906
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/abbs/gmr116
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