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A generic selection system for improved expression and thermostability of G protein-coupled receptors by directed evolution

Structural and biophysical studies as well as drug screening approaches on G protein-coupled receptors (GPCRs) have been largely hampered by the poor biophysical properties and low expression yields of this largest class of integral membrane proteins. Thermostabilisation of GPCRs by introduction of...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Klenk, Christoph, Ehrenmann, Janosch, Schütz, Marco, Plückthun, Andreas
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4758055/
https://ncbi.nlm.nih.gov/pubmed/26887595
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep21294
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