Site-specific protein modification using immobilized sortase in batch and continuous-flow systems

Transpeptidation catalyzed by sortase A allows the preparation of proteins that are site-specifically and homogeneously modified with a wide variety of functional groups, such as fluorophores, PEG moieties, lipids, glycans, bioorthogonal reactive groups and affinity handles. This protocol describes...

Descripción completa

Guardado en:
Detalles Bibliográficos
Publicado en:Nat Protoc
Autores principales: Witte, Martin D., Wu, Tongfei, Guimaraes, Carla P., Theile, Christopher S., Blom, Annet E.M., Ingram, Jessica R., Li, Zeyang, Kundrat, Lenka, Goldberg, Shalom D., Ploegh, Hidde L.
Formato: Artigo
Lenguaje:Inglês
Publicado: 2015
Materias:
Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4757899/
https://ncbi.nlm.nih.gov/pubmed/25719269
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2015.026
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!

Ejemplares similares