Site-specific protein modification using immobilized sortase in batch and continuous-flow systems

Transpeptidation catalyzed by sortase A allows the preparation of proteins that are site-specifically and homogeneously modified with a wide variety of functional groups, such as fluorophores, PEG moieties, lipids, glycans, bioorthogonal reactive groups and affinity handles. This protocol describes...

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Detaylı Bibliyografya
Yayımlandı:Nat Protoc
Asıl Yazarlar: Witte, Martin D., Wu, Tongfei, Guimaraes, Carla P., Theile, Christopher S., Blom, Annet E.M., Ingram, Jessica R., Li, Zeyang, Kundrat, Lenka, Goldberg, Shalom D., Ploegh, Hidde L.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 2015
Konular:
Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC4757899/
https://ncbi.nlm.nih.gov/pubmed/25719269
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2015.026
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