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Production of unnaturally linked chimeric proteins using a combination of sortase-catalyzed transpeptidation and click chemistry
Chimeric proteins, including bi-specific antibodies, are biological tools with therapeutic applications. Genetic fusion and ligation methods allow the creation of N-to-C and C-toN fused recombinant proteins, but not the majority of non-template encoded fusions. The present protocol describes a simpl...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3975145/ https://ncbi.nlm.nih.gov/pubmed/23989675 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2013.103 |
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