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Site-specific N-terminal labeling of proteins using sortase-mediated reactions
For many proteins, the N- or the C-terminus make essential contributions to substrate binding, for protein-protein interactions, or for anchoring the proteins to a membrane. In other circumstances, at least one of the termini is buried within the protein, rendering it inaccessible to labeling. The p...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3941705/ https://ncbi.nlm.nih.gov/pubmed/23989674 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2013.102 |
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