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Site-specific N-terminal labeling of proteins using sortase-mediated reactions

For many proteins, the N- or the C-terminus make essential contributions to substrate binding, for protein-protein interactions, or for anchoring the proteins to a membrane. In other circumstances, at least one of the termini is buried within the protein, rendering it inaccessible to labeling. The p...

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Bibliografske podrobnosti
Main Authors: Theile, Christopher S., Witte, Martin D., Blom, Annet E.M., Kundrat, Lenka, Ploegh, Hidde L., Guimaraes, Carla P.
Format: Artigo
Jezik:Inglês
Izdano: 2013
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3941705/
https://ncbi.nlm.nih.gov/pubmed/23989674
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2013.102
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