Combination of dextran sulfate and recombinant trypsin on aggregation of Chinese hamster ovary cells

In laboratory scale therapeutical protein production, cell clumps form typically in shake flasks, which hinders cell growth and decreases protein yield. To minimize clumps during the culture of Chinese hamster ovary cells, we employed the combination of two reagents, dextran sulfate (DS) and recombi...

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Detalhes bibliográficos
Publicado no:Cytotechnology
Main Authors: Jing, Yu, Zhang, Cunchao, Fu, Tuo, Jiang, Cheng, Ma, Kai, Zhang, Dapeng, Hou, Sheng, Dai, Jianxin, Wang, Hao, Zhang, Xueguang, Kou, Geng, Guo, Yajun
Formato: Artigo
Idioma:Inglês
Publicado em: Springer Netherlands 2014
Assuntos:
Method in Cell Science
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4754253/
https://ncbi.nlm.nih.gov/pubmed/25087075
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10616-014-9774-4
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