Combination of dextran sulfate and recombinant trypsin on aggregation of Chinese hamster ovary cells

In laboratory scale therapeutical protein production, cell clumps form typically in shake flasks, which hinders cell growth and decreases protein yield. To minimize clumps during the culture of Chinese hamster ovary cells, we employed the combination of two reagents, dextran sulfate (DS) and recombi...

Täydet tiedot

Tallennettuna:
Bibliografiset tiedot
Julkaisussa:Cytotechnology
Päätekijät: Jing, Yu, Zhang, Cunchao, Fu, Tuo, Jiang, Cheng, Ma, Kai, Zhang, Dapeng, Hou, Sheng, Dai, Jianxin, Wang, Hao, Zhang, Xueguang, Kou, Geng, Guo, Yajun
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: Springer Netherlands 2014
Aiheet:
Method in Cell Science
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC4754253/
https://ncbi.nlm.nih.gov/pubmed/25087075
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10616-014-9774-4
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